Is it necessasry for me to run Spybot and Adaware or does Comodo protect in these two areas?
Thanks for your help.

Comodo has an antispyware (Ad-Aware) and Defense+ can protect you against registry changes (spybot) so you don’t need to run Ad-Aware or Spybot with it.

But it is advisable to occasionally run a scan with MBAM.